Prof. Dr. Mareike Dirks-Hofmeister

bei Interesse an einer Projekt-, Bachelor- oder Masterarbeit zu den folgenden Themenfeldern, nehmen Sie gerne Kontakt zu mir auf:

∙ mikrobielle/fermentative Verwertung von Abfallströmen ∙ pilzliche Produktionssysteme für Enzyme ∙ Enzyme Engineering ∙ Down-Scaling von Enzymanwendungen ∙ fermentative Produktion funktionelle Inhaltsstoffe ∙

Bachelorarbeit ∙ Untersuchungen zur Fermentierbarkeit verschiedener Zucker in der Herstellung von Kombucha ∙ 06/2021-08/2021

Bachelorarbeit ∙ Planung und Konzeption der Deletion von Mykotoxin-Genclustern in Aspergillus niger CBS115989 mittels CRISPR/Cas ∙ 07/2021-09/2021

Projektarbeit ∙ Optimierung des Kulturmediums von Aspergillus niger zur Produktion von Enzymen ∙ 04/2021-03/2022

Professorin für Lebensmittelbiotechnologie

Fakultät für Agrarwissenschaften und Landschaftsarchitektur

Hochschule Osnabrück

R&D Director

WeissBioTech GmbH, Produktionsstandort Büttelborn

∙ Enzymprodukte für die Lebensmittelindustrie ∙

R&D Project Leader

WeissBioTech GmbH, Forschungsstandort c/o BRAIN AG Zwingenberg

∙ Produktentwicklung ∙ Enzymanwendungen ∙ Fördermanagement ∙

Postdoctoral Researcher

Ghent University, Centre for industrial Biotechnology and Biocatalysts (InBio.be)

∙ FP7-project NOVOSIDES ∙ Enzyme Engineering ∙ enzymatische Glykosylierung pflanzlicher Polyphenole ∙

Postdoktorandin (Gruppenleitung)

WWU Münster, Institut für Biochemie und Biotechnologie der Pflanzen (IBBP)

∙ Polyphenoloxidasen ∙ Struktur-Funktions-Beziehungen von Enzymen ∙

Promotion zur Dr. rer. nat.

WWU Münster, Institut für Biochemie und Biotechnologie der Pflanzen (IBBP)

Thema: Structural and functional diversity of the polyphenol oxidase family in dandelion (Taraxacum officinale)

Studium

WWU Münster

∙ MSc Biology (2007) ∙ BSc Biowissenschaften (2005) ∙

FunChi – Fungal Chitosans from Fermentation Mycelia for Plant Biostimulants

ERA-IB 2: 6^th call

Partner: WeissBioTech GmbH, WWU Münster, Leiden University (NL), Dutch DNA Biotech (NL), Universidad Polytecnica de Madrid (ES), Plant Response Biotech (ES)

F2F – From Fungal Biomass to Fine Chemicals

ZIM-BMWi

Partner: WeissBioTech GmbH, IBBP - WWU Münster, IMMB - WWU Münster, CeBiTec - Universität Bielefeld, Cysal GmbH

Filazyme - Novel approaches to develop filamentous microorganism for enzyme production

ERA-IB: 5^th call

Partner: WeissBioTech GmbH, Leiden University (NL), Dutch DNA Biotech (NL), Salamanca University (ES), H2Biyotek (TR)

Proof of concept for antioxidant glycosylation - Exploring the industrial potential of phenolic glycosides produced with a newly developed enzyme variant

StarTT IOF - UGent

Ghent University (BE)

Funktionen von Polyphenoloxidasen im Löwenzahn (Taraxacum officinale) - Voraussetzung für eine wirtschaftlich nachhaltige Nutzung

Promotionsstipendium

Deutschen Bundesstiftung Umwelt (DBU)

De Bie J, Dirks-Hofmeister ME, Pelzer A, Klermund L (priority 2019-12-13): Process for the preparation of a fermentable sugar composition and the fermentation thereof. WO 2021/116400.

Vortmann M, Stumpf AK, Sgobba E, Dirks-Hofmeister ME, Krehenbrink M, Wendisch VF, Philipp B, Moerschbacher BM (2021): A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine, Applied Microbiology and Biotechnology, https://doi.org/10.1007/s00253-021-11112-5.

Prexler SM, Frassek M, Moerschbacher BM, Dirks-Hofmeister ME (2019): Catechol Oxidase versus Tyrosinase Classification Revisited by Site-Directed Mutagenesis Studies, Angewandte Chemie International Edition 58: 8757-8761, https://doi.org/10.1002/anie.201902846.

Stumpf AK, Vortmann M, Dirks-Hofmeister ME, Moerschbacher BM, Philipp B (2019): Identification of a novel chitinase from Aeromonas hydrophila AH-1N for the degradation of chitin within fungal mycelium, FEMS Microbiology Letters 366: fny294, https://doi.org/10.1093/femsle/fny294.

Sgobba E, Stumpf AK, Vortmann M, Jagmann N, Krehenbrink M, Dirks-Hofmeister ME, Moerschbacher BM, Philipp B, Wendisch VFF (2018): Synthetic Escherichia coli - Corynebacterium glutamicum consortia for L-Lysine production from starch and sucrose, Bioresource Technology 260: 302-310, https://doi.org/10.1016/j.biortech.2018.03.113.

Prexler SM, Singh R, Moerschbacher BM, Dirks-Hofmeister ME (2017): A specific amino acid residue in the catalytic site of dandelion polyphenol oxidases acts as 'selector' for substrate specificity, Plant Molecular Biology 96(1-2): 151-164, https://doi.org/10.1007/s11103-017-0686-5.

Desmet T, Dirks-Hofmeister ME, De Winter K, Verhaeghe T (priority 2015-05-12): Mutant sucrose phosphorylases with improved glycosylation activity towards polyphenols. WO 2016/180818 A1.

De Winter K, Dewitte G, Dirks-Hofmeister ME, De Laet S, Pelantová H, Krên V, Desmet T (2015): Enzymatic glycosylation of phenolic antioxidants: Phosphorylase-mediated synthesis and characterization, Journal of Agricultural and Food Chemistry 63: 10131-9, https://doi.org/10.1021/acs.jafc.5b04380.

Dirks-Hofmeister ME, Verhaeghe T, De Winter K, Desmet T (2015): Creating Space for Large Acceptors: Rational Biocatalyst Design for Resveratrol Glycosylation in an Aqueous System, Angewandte Chemie International Edition 54(32): 9289-92, https://doi.org/10.1002/anie.201503605.

Leufken CM, Moerschbacher BM, Dirks-Hofmeister ME (2015): Dandelion PPO-1/PPO-2 domain-swaps: the C-terminal domain modulates the pH optimum and the linker affects SDS-mediated activation and stability, Biochimica Biophysica Acta 1854(2):178-186, https://doi.org/10.1016/j.bbapap.2014.11.007.

Dirks-Hofmeister ME, Singh R, Leufken CM, Inlow JK, Moerschbacher BM (2014): Structural diversity in the dandelion (Taraxacum officinale) polyphenol oxidase family results in different responses to model substrates, PLoS ONE 9(6): e99759, https://doi.org/10.1371/journal.pone.0099759.

Dirks-Hofmeister ME, Kolkenbrock S, Moerschbacher BM (2013): Parameters that enhance the bacterial expression of active plant polyphenol oxidases, PLoS ONE 8(10): e7791, https://doi.org/10.1371/journal.pone.0077291.

Dirks-Hofmeister ME, Inlow JK, Moerschbacher BM (2012): Site-directed mutagenesis of a tetrameric dandelion polyphenol oxidase (PPO-6) reveals the site of subunit interaction, Plant Molecular Biology 80, 203-217, https://doi.org/10.1007/s11103-012-9943-9.

Richter C, Dirks ME, Schulze Gronover C, Prüfer D, Moerschbacher BM (2012): Silencing and heterologous expression of ppo-2 indicate a specific function of a single polyphenol oxidase isoform in resistance of dandelion (Taraxacum officinale) against Pseudomonas syringae pv. tomato, Molecular Plant-Microbe Interactions 25, 200-210, https://doi.org/10.1094/MPMI-04-11-0082.